Rates of intercalator-driven platination of DNA determined by a restriction enzyme cleavage inhibition assay

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases.

We have developed a rapid and high-throughput assay based on rolling circle amplification, to distinguish individual strand cleavage of DNA duplexes by restriction endonucleases. As an illustration, we analyzed nicking activity of Nb.BbvCI and uneven cleavage of LNA modified DNA by EcoRI. This assay has potential for analyzing protein-DNA interactions.

An Assay for the Rates of Cleavage of Specific Sites in DNA by Restriction Endonucleases: Its Use to Study the Cleavage of Phage A DNA by EcoRl and Phage P22 DNA Containing Thymine or 5Bromouracil by HindIll

A method to measure the rates of cleavage of specific sites in DNAs by restriction endonucleases is described. Partial digests are prepared by incubating DNAs with limiting amounts of endonuclease. The termini generated by cleavage are labeled with “P by the polynucleotide kmaseexchange reaction. The labeled termini are then identified by completing the digestion with the same endonuclease and ...

Specificity of Cryptococcus neoformans factor sera determined by enzyme-linked immunosorbent assay and dot enzyme assay.

An indirect enzyme-linked immunosorbent assay (ELISA) and a dot enzyme assay (DEA) were used to determine the specificities of Cryptococcus neoformans factor sera to serotype type-specific capsular polysaccharides, glucuronoxylomannans (GXMs). Pure and chemically characterized GXMs were obtained from representative isolates of C. neoformans serotypes A, B, C, and D. Distinctive specificity patt...

Cleavage of mispaired heteroduplex DNA substrates by numerous restriction enzymes.

The utility of restriction endonucleases as a tool in molecular biology is in large part due to the high degree of specificity with which they cleave well-characterized DNA recognition sequences. The specificity of restriction endonucleases is not absolute, yet many commonly used assays of biological phenomena and contemporary molecular biology techniques rely on the premise that restriction en...